ABSTRACT This study evaluated the behavior of broiler chickens subjected to thermal stress during different periods of the day and age of birds. A total of 180 one-day-old male broiler chicks were allocated to three chambers. Each chamber contained five boxes, with 12 chicks/box. Birds were assigned to a completely randomized design. The birds were filmed, and the images recorded every minute during the two periods: morning (from 08:00 to 09:00 h) and afternoon (from 17:00 to 18:00 h), at 7, 14, and 35 d of age. The frequency of the behavioral category (water intake, feed intake, resting, exploration, and comfort) was determined. Data were subjected to variance analysis for mixed models with rearing temperatures (cold, usual, and hot) and age of birds (7, 14, and 35 d) as subdivided plot, and periods of the day (morning and afternoon) as sub-subdivided plot. Birds at cold and usual temperatures showed higher feed intake than those at hot temperature in the morning. Birds exposed to cold and usual temperatures explored the environment less frequently at 35 d when compared with 14 d. However, chickens at hot temperature showed decreased exploration according to the increase of age. Birds spent more time feeding during the morning, regardless of age and rearing temperature. Also, during this period of the day, chickens spent most of the time exploring the environment. The frequency of feed intake and exploration decreases with the increase of age. The similarity of behavior between chickens reared at cold and usual temperatures may be due to a change in the thermal comfort zone of the birds. It suggests that the real thermal comfort temperature is between the two temperature ranges studied (cold and usual).

Resumo: Genótipos mais produtivos da cultura da soja é um dos principais objetivos dos programas de melhoramento. Existem poucos estudos quanto à seleção de caracteres associados à qualidade e desempenho de sementes de soja. Objetivou-se predizer parâmetros fisiológicos e genéticos sobre a qualidade de sementes produzidas em populações segregantes de soja, provenientes de genitores com diferentes grupos de maturidade. Foram avaliados oito genótipos da população segregante F2: 3 de soja (163, 24, 57, 108, 164, 169, 157 e 81), cultivados na safra 2018/19. Para a condução do experimento foi utilizado o delineamento inteiramente casualizado. Avaliou-se a qualidade fisiológica das sementes por meio dos testes de germinação, tetrazólio e análise de imagem. As análises estatísticas foram realizadas utilizando-se uma abordagem de modelos mistos. Foi possível observar maior vigor nas sementes dos lotes 108, 164 e 169, cujos resultados estiveram entre os melhores na maioria dos parâmetros analisados. Maior incidência de dano mecânico e por percevejos pode ser usado para explicar o menor vigor, como observado nos genótipos 57 e 163, enquanto o dano por umidade não corroborou com os testes de vigor das sementes. A resposta dos genótipos às análises realizadas indicou maior influência de características genéticas destes do que efeito do grupo de maturidade relativa. Não foi possível observar evidente efeito do grupo de maturidade relativa e o vigor das sementes de soja.

Abstract: Productive soybean genotypes is one of the main objectives of soybean breeding programs. However, there are few studies on the selection of characteristics associated with the quality and performance of soybean seeds. The objective in this study was to predict physiological and genetic parameters associated with seed quality in segregating soybean populations derived from parents of differing maturity groups. Eight genotypes of the F2:3 segregating soybean population (numbered 163, 24, 57, 108, 164, 169, 157, and 81) cultivated in the 2018/19 harvest were evaluated. To conduct the experiment, a completely randomized design was used. The physiological quality of the seeds was evaluated by germination test, tetrazolium test, and image analysis. Statistical analyses were performed by using a mixed model approach. Greater seed vigor was observed in the genotypes 108, 164, and 169, whose results were among the best for most of the analyzed parameters. The greater mechanical damage and damage due to stink bugs can explain the lower vigor of some seeds, as observed in genotypes 57 and 163, while weathering damage did not correspond to the results of the seed vigor tests. The analyses indicated a greater influence of genetic characteristics than of relative maturity group on the responses of the genotypes. No effect of relative maturity group on soybean seed vigor was evident.

ABSTRACT Different levels of glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C were injected in ovo to determine whether additive use influences the incubation parameters and bone characteristics of posthatch chicks. Hatchability was not affected by addition of glycosaminoglycans and vitamin C in ovo. However, chicks that received 4 μg additive showed 2.86% reduction in total mortality when compared with chicks from non-injected eggs. Moreover, tibia area and femur bone mineral density increased in chicks from eggs injected with 2.16 and 6.00 μg of additive, respectively. In ovo feeding with glycosaminoglycans and vitamin C can benefit bone development in embryos and reduce total mortality during the incubation period.

Lapachol was chemically modified to obtain its thiosemicarbazone and semicarbazone derivatives. These compounds were tested for antimicrobial activity against several bacteria and fungi by the broth microdilution method. The thiosemicarbazone and semicarbazone derivatives of lapachol exhibited antimicrobial activity against the bacteria Enterococcus faecalis and Staphylococcus aureus with minimal inhibitory concentrations (MICs) of 0.05 and 0.10 µmol/mL, respectively. The thiosemicarbazone and semicarbazone derivatives were also active against the pathogenic yeast Cryptococcus gattii (MICs of 0.10 and 0.20 µmol/mL, respectively). In addition, the lapachol thiosemicarbazone derivative was active against 11 clinical isolates of Paracoccidioides brasiliensis, with MICs ranging from 0.01-0.10 µmol/mL. The lapachol-derived thiosemicarbazone was not cytotoxic to normal cells at the concentrations that were active against fungi and bacteria. We synthesised, for the first time, thiosemicarbazone and semicarbazone derivatives of lapachol. The MICs for the lapachol-derived thiosemicarbazone against S. aureus, E. faecalis, C. gattii and several isolates of P. brasiliensis indicated that this compound has the potential to be developed into novel drugs to treat infections caused these microbes.

O Programa de Saúde da Família (PSF) surgiu como estratégia de reorientação da atenção básica em saúde. A incorporação de profissionais de saúde mental no PSF não foi prevista na Portaria nº 648 (2006), que definiu os recursos mínimos para a atuação das equipes. O Ministério da Saúde, em 2007, aprovou os Núcleos de Atenção à Saúde da Família. A Residência Multiprofissional em Saúde da Família foi uma proposta coordenada pela Faculdade e Casa de Saúde Santa Marcelina, contando com dez categorias profissionais, entre elas a psicologia. Este trabalho tem o objetivo de refletir sobre a prática do psicólogo residente e analisá-la, contribuindo para a discussão das possibilidades de atuação do psicólogo no PSF na cidade de São Paulo. Trata-se de uma pesquisa avaliativa do tipo estudo de caso, que tem o objetivo de sistematizar as intervenções realizadas, o processo de construção da planilha mensal de produção e alguns dados que ela fornece. Os resultados são: a descrição das categorias, a planilha mensal de produção com adaptações a partir dos códigos do Sistema de Informação Ambulatorial (SIA) atribuídos aos psicólogos e um quadro com os dados quantitativos. Foi possível perceber que os princípios do PSF, a formação em psicologia e a vinculação com a Residência estão relacionados com os caminhos percorridos. Concluiu-se, então, que a quantificação é necessária para a visualização das ações, entretanto, não é suficiente para avaliar a abrangência, a eficácia e a efetividade das ações e o quanto o SIA não está preparado para o registro, o controle e a fiscalização do psicólogo inserido no PSF.

The Family Health Program (FHP) emerged in Brazil as a strategy of basic health care reorientation. The incorporation of Mental Health professionals in the FHP was not included in Directive 648 (year?), which defines minimum resources for the teams' work. In 2007, the Brazilian Ministry of Health approved the Family Healthcare Nuclei. The Multiprofessional Family Health Residency was a proposal that was coordinated by the Santa Marcelina Faculty and Hospital. It had ten professional categories, one of them being the psychologists. This work aims to reflect on and analyze the resident psychologists' practice and contribute to the discussion about the possibilities for the psychologists' work in the FHP in the city of São Paulo. It is a descriptive research; more specifically, a case study. The objective is the systematization of the practices, of the process of construction of the monthly production table and of some data it provides. The results are: categories description, the monthly production table, with some adaptations based on the codes of the Day-Care Information System that were attributed to psychologists, and a framework with quantitative data. It was possible to notice that: FHP principles, having attended a psychology undergraduation course and being part of the residency program are related with the psychologists' own history. The conclusion is that, although quantifying is necessary for the understanding of the practices, it is not sufficient to assess the complexity, efficacy and effectiveness of the practices. In addition, quantifying does not reveal the extent to which the Day-Care Information System is not prepared to register, control and inspect the psychologists who work in the FHP.

O Programa de Saúde da Família (PSF) surgiu como estratégia de reorientação da atenção básica em saúde. A incorporação de profissionais de saúde mental no PSF não foi prevista na Portaria nº 648 (2006), que definiu os recursos mínimos para a atuação das equipes. O Ministério da Saúde, em 2007, aprovou os Núcleos de Atenção à Saúde da Família. A Residência Multiprofissional em Saúde da Família foi uma proposta coordenada pela Faculdade e Casa de Saúde Santa Marcelina, contando com dez categorias profissionais, entre elas a psicologia. Este trabalho tem o objetivo de refletir sobre a prática do psicólogo residente e analisá-la, contribuindo para a discussão das possibilidades de atuação do psicólogo no PSF na cidade de São Paulo. Trata-se de uma pesquisa avaliativa do tipo estudo de caso, que tem o objetivo de sistematizar as intervenções realizadas, o processo de construção da planilha mensal de produção e alguns dados que ela fornece. Os resultados são: a descrição das categorias, a planilha mensal de produção com adaptações a partir dos códigos do Sistema de Informação Ambulatorial (SIA) atribuídos aos psicólogos e um quadro com os dados quantitativos. Foi possível perceber que os princípios do PSF, a formação em psicologia e a vinculação com a Residência estão relacionados com os caminhos percorridos. Concluiu-se, então, que a quantificação é necessária para a visualização das ações, entretanto, não é suficiente para avaliar a abrangência, a eficácia e a efetividade das ações e o quanto o SIA não está preparado para o registro, o controle e a fiscalização do psicólogo inserido no PSF.

The Family Health Program (FHP) emerged in Brazil as a strategy of basic health care reorientation. The incorporation of Mental Health professionals in the FHP was not included in Directive 648 (year?), which defines minimum resources for the teams' work. In 2007, the Brazilian Ministry of Health approved the Family Healthcare Nuclei. The Multiprofessional Family Health Residency was a proposal that was coordinated by the Santa Marcelina Faculty and Hospital. It had ten professional categories, one of them being the psychologists. This work aims to reflect on and analyze the resident psychologists' practice and contribute to the discussion about the possibilities for the psychologists' work in the FHP in the city of São Paulo. It is a descriptive research; more specifically, a case study. The objective is the systematization of the practices, of the process of construction of the monthly production table and of some data it provides. The results are: categories description, the monthly production table, with some adaptations based on the codes of the Day-Care Information System that were attributed to psychologists, and a framework with quantitative data. It was possible to notice that: FHP principles, having attended a psychology undergraduation course and being part of the residency program are related with the psychologists' own history. The conclusion is that, although quantifying is necessary for the understanding of the practices, it is not sufficient to assess the complexity, efficacy and effectiveness of the practices. In addition, quantifying does not reveal the extent to which the Day-Care Information System is not prepared to register, control and inspect the psychologists who work in the FHP.

Os níveis de citocinas e síntese intratecal de IgG foram dosados no líquido cefalorraquidiano (LCR) e soro, com o objetivo de avaliar a atividade inflamatória em pacientes com esclerose múltipla durante remissão clínica. Foram detectados níveis elevados de citocinas pró-inflamatórias (TNFalfa e IFNgama) no LCR e soro, sem alterações significativas na produção de IL12 e IL10. O perfil de produção das citocinas pró-inflamatórias estava associado ao aumento de leucócitos no LCR, assim como a presença de bandas oligoclonais IgG sugerindo síntese intratecal de IgG. Estes resultados sugerem que mesmo quando a doença está clinicamente silenciosa, a atividade inflamatória está presente nestes pacientes.

Cytokines and intrathecal IgG synthesis were determined in the cerebrospinal fluid (CSF) and sera to evaluate inflammatory activity in multiple sclerosis (MS) patients during clinical remission. Although the disease was stable, there had been a significant increase of proinflammatory cytokines such as TNFalpha and IFNgamma in the CSF and serum, with no significant changes of IL12 and IL10 production. The changes in the cytokine production patterns were associated with an increase of leukocytes in the CSF, as well as the presence of oligoclonal bands suggesting intrathecal IgG synthesis. These results suggest that even when the disease is clinically silent, one can observe inflammatory activity in these MS patients.